HeMoQuest

A webserver for the qualitative prediction of Transient Heme-Binding Motifs

This webpage provides access to HeMoQuest, a computational approach for the prediction of transient heme-binding motifs from protein sequences. HeMoQuest uses the SeqD-HBM^[2] algorithm to detect transient heme-binding motifs and a machine learning approach trained on in-house experimental data to predict the heme-binding affinities of the individual motifs.

If you use HeMoQuest in any part of your work, please cite the following publications:

Paul George, A.A., Lacerda, M., Syllwasschy, B.F., Hopp, M.-T., Wißbrock, A., Imhof, D. (2020) HeMoQuest: A webserver for qualitative prediction of transient heme binding to protein motifs. BMC Bioinformatics, 21, 124
Wißbrock, A., Paul George, A.A., Brewitz, H. H., Kühl, T., Imhof, D. (2019) The Molecular Basis of Transient Heme-Protein Interactions: Analysis, Concept and Implementation. Biosci. Rep. 39(1), BSR20181940

If you use HeMoQuest with WESA to perform solvent accessibility checks on your sequence please cite the relevant literature as well.

Chen, H-L and Zhou, H-X. Prediction of solvent accessibility and sites of deleterious mutations from protein sequence. Nucl. Acids Res. 33, 3193-3199 (2005).
Shan, Y., Wang, G., and Zhou, H.-X. Fold recognition and accurate query-template alignment by a combination of PSI-BLAST and threading. Proteins 42, 23-37 (2001).

Further enhancements to the web interface and the underlying logic are a work in progress.

If our research interests you, please visit our homepage.

HeMoQuest User Input

Enter a valid email address to receive the complete results for the submitted sequence(s). Email entry is MANDATORY in cases where WESA solvent accessibility predictions are requested:

Select a local fasta file. Fasta files can contain multiple sequences placed one below the other provided, individual sequences are separated by valid fasta headers:

Enter your sequence in standard amino acid 1-letter codes without fasta headers. Only the 20 standard amino acids are accepted:

Run WESA solvent accessibility prediction to discard buried residues ?

No, don't run WESA

Yes, run WESA (Works for only sequences under 2000 residues and requires 10-30 minutes per sequence for processing !)

Please note : After clicking the submit button, please wait to be automatically redirected to the results page!

HeMoQuest Datasets

All datasets used in the development and testing of HeMoQuest are available for download here.

HeMoQuest Support

For queries regarding HeMoQuest, the usage of this site or about submitted jobs, please write to us at: help.hemoquest@gmail.com.

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